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Conventional microbiological methods were used for the selective isolation of the major microfouling bacteria.
Results of conventional microbiological methods were used to determine the sensitivity and specificity of the multiplex real-time PCR procedure.
The microbiological methods were similar in the two centers (same swab, same medium, same inoculum device and same antibiotic susceptibility testing).
Mainly, amoxicillin (the most abundant residue in the studied milk) and ampicillin were found in half of the samples; which was still less than penicillin (97%%) and/or tetracycline (88%%) as reported by Ben-Mahdi and Ouslimani (2009), and Titouche et al. (2013), respectively where standard microbiological methods were used.
Thus, a series of microbiological methods were utilized to propagate inocula from the above specimens.
For Salmonella, microbiological methods were used for cultivation and isolation, and for serotype identification, as described elsewhere [74].
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An increasing number of molecular techniques for the diagnosis of fungal infections have been developed in the last few years, due to the growing prevalence of mycoses and the length of time required for diagnosis when classical microbiological methods are used.
Molecular microbiological methods are applied in ASKURIS in order to investigate the occurrence of antibiotic-resistance genes[40].
Detailed microbiological methods are published elsewhere (In Press).
Clinical molecular microbiological methods are increasingly being employed to investigate the microbiota of chronic infections, including wounds, as part of standard patient care.
Although the microbiological methods are very sensitive, it is not clear that they possess sufficient accuracy and precision for routine assessment of minor folate species.
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