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In this case, no microbiological medium was used.
Our work suggested usage of PAM for efficient qualitative screening and as a microbiological medium for preferential selection of PAOs on Petri-plates.
Experiments carried out based on the Plackett Burman design demonstrated the advisability of adding the following components to the microbiological medium: MgCO3, K2HPO4, glycerol, urea and whey permeate.
Prior to inoculation, the overnight cultures were centrifuged, and the bacterial biomass obtained was first suspended in a portion (5%) of the adequate tested microbiological medium and then combined with the rest.
The studies were performed for selected species of moulds, which were inoculated onto model samples of silk, cellulose, parchment and wool that had been prepared on microbiological medium, in vials for headspace sampling.
The obtained data show that all the species of mould emitted volatile compounds belonging to the C8 complex, regardless of the kind of material and microbiological medium on which they were cultivated.
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Finally, we managed to establish optimal composition of microbiological culture medium for the quantity of bacterial biomass produced.
The industrial implementation of this process would also require development of a microbiological culture medium based on cheap and easily-available substrates.
The aseptic processing of operators must also be confirmed by performing media fills (the performance of an aseptic manufacturing procedure using a sterile microbiological growth medium in place of the drug solution).
It must be mentioned, that the choice of specific selective or non-selective microbiological growth medium, the incubation temperature and the cultivation time result to differences on the selection of the viable, cultivable, airborne microbial community.
The objective of this study was, therefore, to develop microbiological culture medium, based on cheap and easily available substrates (waste glycerol and whey permeate), dedicated for the succinic acid production with Enterobacter sp. LU1.
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