To compare the microbiological yield of corneal ulcer cultures established by direct inoculation of culture media vs indirect inoculation by means of transport medium (Amies without charcoal).
Mice immunosupressed under this protocol displayed clinical and microbiological patterns of progressive and lethal infectious diseases after inoculation in different organs with diverse human pathogens.
The multi-faceted approach resulted in substantial improvement in the microbiological detection and isolation of Bartonella when compared to direct inoculation of a blood agar plate.
Samples and media preparation, inoculation and colony counts were done using standard microbiological methods (Buckle et al. 1989; Bartram and Balance 1996; FAO 2004).
The use of a beadmill has previously been shown to be highly effective in the microbiological identification of PJI, using multiple peri-prosthetic samples, with inoculation onto solid and liquid media.
The microbiological approach includes the use of non-GMO (genetically modified organisms) strategies like sequential inoculations and co-inoculations of S. cerevisiae with non-Saccharomyces yeasts, as well as GMO-based strategies with the use of genetically modified yeasts.
24 h post-inoculation samples of spleen, ileum and liver were collected for microbiological studies.
Subsequently, in the inter-group comparison of the PET data, the difference between S. aureus group and group 3 without bacterial inoculation was not statistically significant, even when the animals with the negative microbiological results were excluded from the analysis.
The microbiological diagnosis of respiratory tract infections requires serial manual dilutions of the clinical specimen before agar plate inoculation, disrupting the workflow in bacteriology clinical laboratories.
Microbiological culture assays detected infection in only 5 of 7 animals receiving 1 × 104 CFU inoculations.
