The number of cells in microcolonies as a function of incubation time was determined using two methods: scanning electron microscopy (SEM) and microbiological determination of the number of cells washed off of membranes containing known numbers of microcolonies.
Biopsies were taken three times a week for microbiological determination.
The microbiological determination revealed the absence of CFU in the majority of batches and a contamination at the upper end of the capsule specification of ≤1,000 CFU in two batches.
For most microbiological applications, accurate determination of the contact point (zero separation distance) between the AFM tip and the soft sample is rather delicate due to the complex contributions of surface forces and mechanical deformation [9].
Every 3 mo, samples were taken for microbiological testing, reactive sulfhydryl determinations, active enzyme analysis, instrumental viscosity readings, color measurements, and descriptive sensory evaluation.
In order to determine the prevalence of subclinical mastitis, each selected farm was visited once between September and December 1998 and individual quarter milk samples from selected lactating cows were collected for microbiological culture and SCC determinations.
This created challenges in Study 116 for the collection and alignment of available microbiological data for analysis and determination of bacteriological outcomes (i.e. cure versus treatment completion) as defined by WHO.
At admission and three times a week, we took samples of CSF for cytological and biochemical analysis, microbiological qualitative culture and sTREM-1 determination.
It was studied against three bacterial strains, that is, Salmonella typhi, Pseudomonas aeruginosa, and E. coli, using cylinder plate method of microbiological assay (used for the potency determination of antibiotics) [ 30].
As in clinical practice, these determinations were not always supported by microbiological evidence.
