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The number of cells in microcolonies as a function of incubation time was determined using two methods: scanning electron microscopy (SEM) and microbiological determination of the number of cells washed off of membranes containing known numbers of microcolonies.
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The microbiological determination revealed the absence of CFU in the majority of batches and a contamination at the upper end of the capsule specification of ≤1,000 CFU in two batches.
Biopsies were taken three times a week for microbiological determination.
Thus, we conducted several analyses involving physicochemical parameters that exhibited no significant correlation with microbiological determinations.
For most microbiological applications, accurate determination of the contact point (zero separation distance) between the AFM tip and the soft sample is rather delicate due to the complex contributions of surface forces and mechanical deformation [9].
This created challenges in Study 116 for the collection and alignment of available microbiological data for analysis and determination of bacteriological outcomes (i.e. cure versus treatment completion) as defined by WHO.
Microbiological assay׳s overall uncertainty (3.1%) was comparable to those obtained for other microbiological assays (1.7 7.1%) and for determination of linezolid by spectrophotometry (2.1%) and reverse-phase ultra-performance liquid chromatography (RP-UPLC) (2.5%).
It was studied against three bacterial strains, that is, Salmonella typhi, Pseudomonas aeruginosa, and E. coli, using cylinder plate method of microbiological assay (used for the potency determination of antibiotics) [ 30].
In particular, the control of the microbiological spoilage by combined use of three antimicrobials, and the determination of their optimal composition to extend the fish hamburger Microbiological Stability Limit (MAL) are the main objectives of this work.
Secondary objectives included determination of infection bacteriological profiles and microbiological resolution rates.
The samples were sent to the microbiological laboratory at Skåne University Hospital, Lund for culture and determination of antibiotic resistance patterns.
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