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Microbiological analysis was also performed on milk samples obtained from clinically affected mammary glands (n = 59).
Microbiological analysis was performed by inoculating juice with Escherichia coli K12 and counting microbial populations pre- and post-processing.
Fibrous tissues in the fracture gap and devitalised bone fragments were removed (subsequent microbiological analysis was negative for infection).
Microbiological analysis was done in accordance with American Public Health Association (APHA) (2008) standard procedures for analysis.
The samples were transported to the laboratory in a cooler box and the microbiological analysis was carried out within 2 h of collection.
Microbiological analysis was carried out within 3 h after sampling so that the microbiological parameters did not change with time (AWWA 1995).
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Biological specimens for microbiological analysis are often collected in BD Vacutainers®, which are not specifically designed for microbial recovery.
Water samples for microbiological analysis were taken 2 3 m from bank in 100 mm depth.
All media used for microbiological analysis were prepared as indicated by the manufacturer.
Samples for microbiological analysis were stored at 4 °C before commencement of analysis and samples for physiochemical tests were analyzed immediately.
Because infection is an occasional mimicker of bone tumours, systematically sending one or two samples for microbiological analysis is a good practice in uncertain cases [29].
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