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Federally funded multi-site comparative study with multiple microbial targets is currently underway.
Initial recognition of numerous microbial targets is a consequence of electrostatic interactions between the defensins arginine residues and the negatively charged phospholipids of the microbial cytoplasmic membrane [ 2, 5].
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However, it needs to be emphasized that E. coli used in this study as a microbial target was a very sensitive strain; and, therefore, the antimicrobial efficacy of copper nanoparticles would likely be less profound for a typical foodborne pathogen such as E. coli O157 H7.
MassTag PCR is a multiplex assay in which microbial gene targets are coded by a library of 64 distinct mass tags.
To address the need for sensitive multiplex assays in diagnostic molecular microbiology, we created a polymerase chain reaction (PCR) platform in which microbial gene targets are coded by a library of 64 distinct Masscode tags (Qiagen Masscode technology, Qiagen, Hilden, Germany).
The application of multiplex MC-PCR has been concentrated in microbial genotyping where unique targets were identified for differential detection.
Among its microbial target structures, there are bacterial capsular polysaccharides, lipoteichoic acids and 1,3-β-glucans of fungal origin (reviewed by Kilpatrick and Chalmers [ 1]).
The specific antimicrobial activity revealed by the qualitative assay is demonstrating that our compounds are interacting differently with the microbial targets, probably due to the differences in the microbial wall structures.
Isolation and separation of the target microbial cells is quite important in applied microbiology.
In this classical assay, a target microbial strain is uniformly seeded over the surface of a nutrient agar plate.
The specific antimicrobial activity revealed by the qualitative assay demonstrates that our compounds are interacting differently with the microbial targets, probably due to the differences in the microbial wall structures.
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