Sentence examples for microbial suspensions were from inspiring English sources

The test microbial suspensions were spread on Petri dishes and freshly prepared Ag and AuNPs samples were introduced.

Suitable concentrations of microbial suspensions were prepared from (1 for bacteria to 3 for yeast and fungi -day-old liquid stock cultures in cubated on a rotary shaker (100 rpm).

Aliquots of 10 µl of the microbial suspensions were applied to microscope slides, and binding of FITC-labeled Vg to the microbial cells was observed under an Olympus BX51 fluorescence microscope.

In parallel, the microbial suspensions were examined using quantitative real-time PCR and propidium monoazide (PMA, Biotium Inc., Hayward, CA) to quantify only cells with intact membrane (viable cells) as detailed by Nocker et al. [35].

Overnight grown microbial suspensions were standardized to approximately 105 cells/mL [ 36].

The microbial suspensions were further diluted (1 100) in media to obtain a final inoculum of approximately 1.5 × 10 cfu/ml.

After PMA treatment, the genomic DNA of treated microbial suspensions was extracted and purified using the MasterPure DNA purification kit (Epicenter Technologies).

Then 20 μl of each microbial suspension was added into the 4-mm-diameter wells made in the blood agar.

50 µl of microbial suspension was added to each corresponding well of a 96-well microtiter plate (polypropylene) together with 50 µl of peptide solution.

An aliquot of microbial suspension was collected and treated over 24 h with 1/100 ACV, washed with PBS, resuspended in PBS and then treated with 1 mL of ice-cold acetone.

The 106 CFU/mL microbial suspension was then added to each tube containing the glass slide pieces.