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Microbial richness was not sampled during the experiment to minimize stress to the salamanders that would be caused by additional swabbing.
However, the use of identical antibiotic bath protocols in the preliminary and main experiments insured that microbial richness was reduced in the main experiment.
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In all three designs, biofilm microbial metabolic richness was significantly higher (p < .05) than that of interstitial water.
Biofilm microbial metabolic richness was generally statistically higher than that of interstitial water in both unsaturated (1.4 24 times higher) and saturated (1.2 1.7 times higher) CWs.
Microbial community richness was estimated with Catchall (Bunge et al. 2012), and rarefaction curves were created in QIIME (Caporaso et al. 2010).
Microbial gene richness was measured by counting the number of genes that are present for a given sample using a downsized count matrix as performed in the original studies.
The authors suggested, in line with Rook et al. [ 30], that the microbial richness might be protective against, not only gastrointestinal pathogens, but also against autoimmune diseases like ulcerative colitis and Crohn's disease.
Moreover, with increasing incubation time, microbial community diversity and richness were significantly recovered in 1% and 2% CaSx treatments compared to the CK.
It is obvious that there are more data and higher microbial richness obtained from BE (BE-1) than from BEY.
If we extend these principles we can suggest this is likely to be true for microbial richness if they too are dispersal limited, and if we can obtain data in the future at the appropriate scale and using techniques that capture the true extent of their diversity.
These contradictory observations suggest that microbial richness may not be a consistent reliable parameter to distinguish eczema infants from non-eczema ones, even after the attempt to minimize non-health related confounders.
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