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Human microbial reconstitution studies using germ-free mice confirmed the predicted association of Christensenellaceae colonization with the lean phenotype.
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Reconstitution studies revealed a mechanistic framework for substrate discrimination based on IpaJ's function as a GTPase "effector" of bacterial origin.
Here we review theoretical and in vitro reconstitution studies that have uncovered some of the key mechanisms by which actin network organization controls contractile tension generation.
Cell-free reconstitution studies demonstrate that polyketide synthases, which are usually involved in the biosynthesis of secondary metabolites, are responsible for generating complex lipids in mycobacteria.
Identification of dominant and protective antigens, and development of robust assays for performance of immune reconstitution studies awaits further studies.
Strong evidence that supports ANT as the primary component of mPTP is provided by reconstitution studies.
Further, they demonstrated that GF mice do not develop oral tolerance, which was restored by microbial reconstitution in neonatal (3 -week -old) mice but not in older mice [ 17].
Such systems have yielded key insights into ERAD previously and provide several attractive features for reconstitution studies.
Reconstitution studies with the LH1 complex have also demonstrated the relationship between N and carotenoid BChl energy transfer.
Reconstitution studies using lipid dequenching assays and endosomal SNAREs have also suggested that SNAREs alone suffice to drive fusion efficiently.
However, little effort has since been devoted to characterising D in more "realistic" model membranes used in reconstitution studies.
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