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In order to explore the changing rule of soil microbial quantity and respiratory capacity under different construction machineries and rolling times, and find the optimal processing conditions, an experiment was set up and a simulation experimental area was chosen, in which we simulated the main types of reclamation in coal mine area.
The soil samples were divided into two parts, one was sieved through a mesh of <2 mm in diameter, and preserved at 4 °C for analysis of microbial quantity and enzymatic activity; the other was naturally air-dried, and sieved through a mesh of <1 mm in diameter for the analysis of soil physical chemical properties.
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In summary, studies that use different approaches and protocols generate different outcomes concerning microbial quantity, diversity, and composition.
Associations between total microbial quantity score and any wheezing were also similar in farms and in rural/suburban areas (P-value = 0.58 for interaction term).
The reason behind the observed association between total microbial quantity score and asthma is unclear.
Further studies in larger cohorts and in different settings are needed to better define the shape of the association between total microbial quantity score and risk of asthma.
Due to the low numbers of children with asthma ever among farmers, interaction tests between total microbial quantity score and farming on asthma ever could not be performed.
The inverted-U-shaped association between total microbial quantity score and asthma ever tended to be stronger after adjusting for the amount of dust (data not shown).
The associations between total microbial quantity score and any wheezing were similar among children who were sensitized against at least one of the tested inhalant or food allergens at the age of 6 years and who were not sensitized (P-value = 0.66 for interaction term).
The microbial quantities (bacteria and fungi) and enzyme activities (catechol 2,3-dioxygenase and ligninolytic enzymes) generally decreased with the increase in HTTs.
The models of total microbial quantity score (ergosterol, endotoxin, and muramic acid) and asthma ever, atopic dermatitis ever, and sensitization to inhalant allergen were carefully tested for 20 additional confounding factors, which have been described earlier (12).
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