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Open image in new window Fig. 3 The change of microbial quantity under different compaction times.
In order to explore the changing rule of soil microbial quantity and respiratory capacity under different construction machineries and rolling times, and find the optimal processing conditions, an experiment was set up and a simulation experimental area was chosen, in which we simulated the main types of reclamation in coal mine area.
The soil samples were divided into two parts, one was sieved through a mesh of <2 mm in diameter, and preserved at 4 °C for analysis of microbial quantity and enzymatic activity; the other was naturally air-dried, and sieved through a mesh of <1 mm in diameter for the analysis of soil physical chemical properties.
Total microbial quantity score had an inverted-U-shaped association with asthma.
The reason behind the observed association between total microbial quantity score and asthma is unclear.
Total microbial quantity scores tended to be inversely associated with respiratory symptoms (Table S2).
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Similar findings were observed with enzyme activities, which had significantly positive correlations with microbial quantities (p < 0.05).
In addition, the microbial quantities are different when using different construction machineries to compact; for the bacteria and actinomycetes, the quantitative size order is: crawler dozer > dump truck.
Microbial quantities had significantly positive correlations with the aliphatic carbon (C) (p < 0.01) but negative correlations with the aromatic C of biochars (p < 0.01).
The microbial quantities (bacteria and fungi) and enzyme activities (catechol 2,3-dioxygenase and ligninolytic enzymes) generally decreased with the increase in HTTs.
In addition, total microbial quantities are closest to the contrast when using dump truck to compact three times and using crawler dozer to compact five times, indicating that microbial activities of those cases are most similar to the natural soil.
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