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The fidelity of the DNA extraction and microbial quantifications were assessed.
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The following quantifications were performed: soil microbial populations by soil dilution plate technique on selective and semi-selective culture media; microbial respiration and microbial biomass by chloroform fumigation-extraction; microbial activity by fluorescein diacetate hydrolysis; and fatty acid methyl ester (FAME) profiles.
All quantifications are from three biological replicates.
Microbial quantification, IVTD and methane were evaluated with the same design but without time as a factor.
The approach described, that of assessing microbial genome inventory and testing putative modular gene expression by mRNA quantification, is likely to remain an important tool in understanding individual microbial contributions within microbial community activities that maintain the Earth's nitrogen balance.
Microbial quantification in the bottles was performed immediately after substrate feeding (time 0), then intermittently until the end of experiment.
Biomass quantification is the most direct way to evaluate the microbial regrowth in distributed water.
Densitometry quantification was achieved using ImageJ software.
Quantification was performed as in Fig. 1d.
DNA quantification was also carried out.
Quantification was performed by using ImageJ software.
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