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In addition, short-chain hydrocarbon degrading bacteria from the CNMR complex, which carry the SDIMO genes, posses several physiological features that make these organisms a more appropriate bioindicator in microbial prospection methods.
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Therefore, the analysis of microbial community composition based on 16S rRNA genes libraries of relative small size, in spite of representing a fundamental method for a general characterization of microbial communities, seems to be inefficient for a more specific application such as the microbial prospection of oil and gas.
Such microorganisms are capable of consuming this gas, supporting the Microbial Prospection of Oil and Gas (MPOG) methodology.
Cross-checking with non-destructive prospection methods such as geophysics and soil sciences allows the evaluation of the percentage of the sites detectable from above ground.
During the preliminary stage of planning restoration activities, geophysical prospection methods can provide the only non-destructive tool to identify the preservation level of such monuments.
In this context, the Microbial Prospection for Oil and Gas (MPOG), developed in Germany and used as a stand-alone technique for detecting microseepages since 1961, is based on the knowledge that oil and gas fields emit a continuous stream of light-hydrocarbon gases towards the Earth's surface [Schumacher 2000; Wagner et al. 2002; Tucker and Hitzman 1996].
Table 1 shows the microbial transformation methods.
Compatibility of the rapid microbial enumeration method with existing microbial identification methods requires the ability to grow visible colonies (which are pure microbial cultures) from microcolonies.
Microbial typing methods provide clinical microbiologists with a fundamental tool for the epidemiological characterization of microbial pathogens by allowing the distinction of diverse organisms of the same species.
In these syndromes, the failure of cultivation-dependent microbial detection methods reveals our ignorance of microbial growth requirements.
Third, the microbial isolation methods employed in all studies were similar.
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