Microbiological examination of rhizosphere soil have revealed that combined use of colloidal solution of nanoparticles of molybdenum (CSNM, 8 mg/l), and microbial preparation for pre-sowing inoculation of chickpea seeds stimulates the development of 'agronomically valuable' microflora.
Numerous experimental data indicate that application of AA and AA-containing microbial preparations as elicitors of plant defense reactions to the phytopathogen infections is a very promising alternative to the use of environmentally dangerous chemical fungicides.
Taking this into account, we considered that an important step is to compare the impact of the traditional techniques of biotechnology (microbial preparation) and application of colloidal solution of metals, as well as the complex use of conventional and nanotechnology on the composition of microbiota of the plant rhizosphere.
The bioassays were performed on microbial preparations obtained after either direct dilution of soil suspensions or prior density-gradient separation of microbial cells from the soil matrix.
The seeds were dampened with water (2% by weight) in control variant, aqueous suspension of microbial preparation, colloidal solution of molybdenum nanoparticles alone and in combination with microbial preparation.
Microbial herbicides can be divided into microbial preparations (microorganisms that control weeds) and microbially derived herbicides.
The trend to conserve energy has led scientists to study the minimal tillage system, to find ways of replacing conventional inorganic fertilizers with natural organic prod ucts or microbial preparations, and to develop new composting methods.
After 36 h, pure colonies (whitish colonial growth) of Bt were verified under a microscope and further grown in liquid media for preparation of pure bacterial cultures containing enough bacterial cells to prepare microbial formulation of Bt.
Analysis of the results showed that oxidative processes are not developed in all variants of the experiment, and in variants with action of microbial preparation (variant 3), but joint action of nanomolybdenum and microbial preparation (variant 4) caused decrease in their activity, as evidenced by the reduction of TBARS in photosynthetic tissues by 15%% (Fig. 1).
Single treatment with CSNM increased the number of nodules by two times, while the treatment of microbial preparation have not significantly affected the number of nodules per plant.
