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This review highlights recent trends in the metabolic engineering of microbial platforms, such as the industrial yeasts Saccharomyces cerevisiae and Yarrowia lipolytica, for the production of renewable fuels.
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Recent progress in exploring various heterologous microbial platforms towards metabolites such as fatty acids, alcohols and alkanes has improved the prospect of advanced biofuel production.
Microbial platforms like the most widely used Escherichia coli and Saccharomyces cerevisiae have been engineered as versatile cell factories for production of diverse and complex value-added chemicals such as phytochemicals, prebiotics, polysaccaharides and poly amino acids.
The microbial platforms that have been proposed will be reviewed with emphasis on yeast strains.
Cyanobacteria are among the most promising microbial platforms for the construction of photosynthetic cell factories.
The utilization of such optimized native efflux pumps will increase productivity of biofuels synthesis and alleviate toxicity and difficulties in production scale-up in current microbial platforms.
The use of engineered microbial platforms for industrial-scale production of high-value terpenes has emerged as a viable approach, especially for the manufacture of pharmaceutical agents, such as artemisinin and taxol [ 9, 48, 51, 52].
For this reason, E. coli and S. cerevisiae are often used microbial platforms in industrial biotechnology.
Development of microbial platforms for the production of pharmaceuticals and other industrial chemicals is an important area of research.
Simple and process-relevant prokaryotic hosts such as Escherichia coli (E. coli) can be utilized as a microbial platform to intracellularly express AMPs rather than using expensive and complex eukaryotic hosts like insect and yeast cells (Sørensen and Mortensen 2005).
"Artemisinin is a hydrocarbon, and we built a microbial platform to produce it," Keasling said.
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