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In contrast, subgingival microbial plaque samples taken from gingival crevices at periodontitis sites showed only minimal levels of UnPG DHC.
We examined blood plasma samples from periodontally healthy subjects, blood plasma samples from subjects with generalized severe destructive periodontal disease (chronic periodontitis), subgingival microbial plaque samples, and normal human brain samples.
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This study revealed grouping of subjects based on the composition of their entire oral microbiomes, which is consistent with previously reported results based on the microbial composition of their subgingival plaque samples [ 47].
Subgingival plaque samples are known to contain microbial organisms as well as desquamated epithelial cells and inflammatory cells, and this combination apparently possesses the capability to partially de-esterify PG DHC lipids.
To find out about the microbial composition of subgingival patient samples, dentists usually have to send plaque samples to a clinical laboratory.
The semiquantification procedure revealed the relative amounts of each test species in dental plaque samples, indicating that this disposable device has great potential in analysis of microbial composition in the oral cavity and intestinal tract, as well as in point-of-care diagnosis of microbiota-associated diseases.
Collecting plaque samples in only 8 sites per mouth might not have adequately represented the microbial profile of the whole mouth.
The hard layer with high content of minerals and fluoride protects the underlying enamel from organic acids produced in dental microbial plaque [28].
In plaque samples that were uniaxially deformed to 50% elongation, WAXS was utilized to demonstrate transverse hard segment orientation relative to the axis of deformation.
The SYBR Green assay, which was simpler than TaqMan assay in its manipulations, was applied to the clinical plaque samples.
Number of independent plaque samples.
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