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It is important to note that the Syn OS-A and Syn OS-B' isolates, the virome and microbial metagenome samples were not collected simultaneously (see above) and since CRISPR arrays are suspected to evolve rapidly to respond to immediate viral attack, it is not surprising that we do not see more high quality sequence matches.
Microbial metagenome samples at Saanich Inlet and along the LineP transect were also collected during LineP cruises (http://www.pac.dfo-mpo.gc.ca/science/oceans/data-donnees/line-p/index-eng.html).html
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As of today, 210 microbial metagenomes sampled from diverse environments such as the ocean, acid mine drainage, soil, bovine rumen and the human body have been reported (http://www.genomesonline.org/).org/
In addition to the taxonomic binning of metagenomes, it is well suited for profiling microbial communities from metagenome samples because it identifies bacterial, archaeal and eukaryotic community members without being affected by varying primer binding strengths, as in marker gene amplification, or copy number variations of marker genes across different taxa.
Indeed, while much of the global microbial metagenome has now been sampled, the same cannot be said for the global viral metagenome [11].
This virome sample was collected the same month as the samples for the microbial metagenome from Mushroom Spring and thirteen months prior to the collection of the Octopus Spring metagenome sample (November, 2004).
The human gut microbial metagenome has recently been studied on samples taken from 124 human individuals [ 10] and shown to be due to a cohort containing over 1000 prevalent species.
Alternatively, this may be due to sampling bias in the microbial metagenome as phage were not specifically enriched at t0, contrary to the t7 sample.
(A ) SUP05 viral presence in Saanich Inlet microbial metagenomes with OMZ sample names bolded.
We investigated the presence of t7 phage in the t0 microbial metagenome and found that EPV1 was the only phage genome sampled at both time points.
Comparison and classification of metagenome samples is one of the major tasks in the study of microbial communities of natural environments or niches on human bodies.
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