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The findings that led to the current technical standards and the developments of microbial measurements and numerical techniques are discussed.
Soil samples for soil microbial measurements were taken from all plots in May 2004, 2006 and 2008.
Further, we thank C.M. Pusch, T. Volovei, T. Keil, A. Roos and C. Böhm for their help during field work and microbial measurements and M. Ackermann, R. Koller, L. Ruess and M. Klier for helpful comments on earlier versions of the manuscript.
All these reasons may explain the lack of consistent associations between reported microbial measurements and health.
Most important, culture-based or non-culture-based microbial measurements used in many studies may not target actual causal factors.
Earlier reviews had found no consistent associations between respiratory or allergic health outcomes and quantitative microbial measurements.
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Microbial measurement endpoints included decomposition activity (mass loss of organic material), respiration on two different substrates (O2 consumption), heterotrophic bacterial abundance (plate counts), and microbial community metabolic profiles (carbon source utilization patterns in Biolog GN microplates).
The SIR microbial measurement is considered a good indicator of the total physiologically active bacteria in soil, which is a proxy of the active microbial biomass [ 35].
If these associations are confirmed in future studies, this will call into question conclusions based on previous evidence that available indoor microbial measurement strategies, especially culture-based assays of air samples, are not informative regarding indoor mold-related health effects (Mendell et al. 2011; World Health Organization 2009).
Unfortunately, due to the lack of microbial pollution measurements in the study plain, we faced with a limitation in selecting the type of the groundwater quality index.
The observed abundance of luminescent distal tips (<1 38 μg calcite/g total sample) is significantly less than the potential calcite precipitation (250 1100 μg calcite/g total sample) indicated by laboratory microbial activity measurements; a finding consistent with previous studies.
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