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We believe that experience of metabolic engineering (ME) of microbial lipids for biofuel production can now be harnessed towards a successful synthesis of microbial GLs for biomedical and other applications.
An increased use of bio- and renewable diesel as replacement for petroleum-derived diesel in recent years has led to increased interest in the production of microbial lipids for fuel use; the aim being to produce lipids in micro-organisms using substrates which do not directly compete with food uses, as plant oils do.
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Compared to plant oil composition, microbial lipids from R. glutinis are of major interest for alternative oil production for biofuel applications.
It was found that fatty acids with 16 and 18 carbon atoms were the majority, and the fatty acid compositional profiles were similar to those of vegetable oils, suggesting that microbial lipids were suitable for biodiesel production [ 30].
Carbohydrates derived from lignocellulosic materials have been used to culture oleaginous yeasts for microbial lipids [ 4- 8].
However, the costs of microbial lipids remain prohibitively high for commercialization.
Realizing the importance of microbial lipids as a potential source for biodiesel, the strain was evaluated for higher biomass production using statistical modelling approach, which resulted in a lipid yield of 0.436 g/g cell dry weight.
Microbial lipids are potential alternative feedstock for biofuel and oleochemical industries.
Considering the demand from many industrial sectors, the production of microbial lipids constitutes an attractive topic for research.
It has been reported that up to 75% of the total costs came from the feedstocks or carbon sources required for producing microbial lipids [ 2].
Microbial lipids have been developed as potential substitutes for high value products, such as cocoa butter and polyunsaturated fatty acids [ 20, 21].
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