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Microbial limitation by C was lowest, suggesting that even microbial C-limitation was constrained by nutrients.
Along these lines, biofilm colonization potential can be inferred by calculating the average conversion efficiency of cellulose during the microbial limitation zone.
The results suggest that cellulose conversion kinetics is initially subject to a microbial limitation period where the substrate is in excess, followed by a substrate limitation period where cellular mass, in the form of biofilms, is not limiting.
As it is seen with our examples, increasing surface availability extends the period of microbial limitation, an aspect that may have to be considered when utilizing microbes with low specific growth, or more appropriately, specific biofilm development rates.
Integration of CO2 production rates over time showed Whatman #3 underwent the fastest conversion efficiency under microbial limitation, suggestive of best biofilm penetration, while Whatman #1 exhibited the least recalcitrance and the faster degradation during the substrate limitation period.
The results showed that the specific biofilm development rate of cellulolytic bacteria such as C. thermocellum has a notable effect on overall reactor kinetics during the period of microbial limitation, when ca. 20% of cellulose conversion occurs.
Similar(53)
Our findings are in line with similar levels of microbial N limitation in arctic, boreal, and temperate ecosystems and suggest that differences in N limitation between these systems were minor compared to differences between soil horizons.
Figure 2 Respiration and microbial nutrient limitation.
Table 3 Regression model results for microbial nutrient limitation Explanatory variable Estimate Std.
Multiple regression analysis revealed the significant explanatory variables for microbial nutrient limitation.
No response of Rs to increased N availability resulting from N input was attributed to microbial C limitation in a semiarid grassland during a 168-hour observation.
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