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"But increasingly, success will come to those who are the most sophisticated about how to do microbial isolation and sequencing and computational biology.
In spite of the well established exploration methods, it is important to emphasize that all of the techniques applied for such goals have been, for many years, based on traditional microbiological methods, encompassing microbial isolation and colony-forming units counting.
The within study bias was assessed on the basis of the qualities of the sampling design, the microbial isolation and identification methods, and the antimicrobial test methods.
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Traditional methods of microbial isolation, development and extraction are now replaced with novel techniques and strategies through biotechnology; with the advent of fermentation technology and genetic engineering techniques.
We employed a variety of insect collection and microbial isolation techniques during year 1 to devise optimal conditions.
Methodology used for food safety determination was the traditional microbial isolation of E. coli O157 H7 and Salmonella.
Improved filtration methods [ 53], electrochemical methods [ 49], immunochemical methods [ 54- 56] and nanotechnologies [ 57, 58] may enable better cell sorting and improve microbial isolation techniques, but each of these has its own challenges to overcome.
Multiple water samples were collected for microbial isolation, water pH was ~ 2 and temperature ranged from 25to5252 °C [ 40] (cooler than SHP).
Soil cores (0 20 cm) taken from selected spots were collected in sterile plastic bags and stored at 4 °C until microbial isolation.
The collected soil samples were brought to the laboratory, dried, and kept at 4 °C until further microbial isolation was performed.
Subsequent targeted microbial isolations from worker and soldier washes and gut contents, as well as freshly deposited and older fungus comb material, were performed during fieldwork collections in South Africa in 2011 and 2013.
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