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Small molecule production in microbial hosts is limited by the accumulation of the product inside the cell.
The relationship between phage and their microbial hosts is difficult to elucidate in complex natural ecosystems.
The development of microbial hosts is still in its infancy stage with process details not published yet.
Based on recent examples from metabolic engineering, we expect that these types of host pathway interactions are common (Kizer et al., 2008; Ro et al., 2008; Verwaal et al., 2010; Michener et al., 2012; Bernhardt and Urlacher, 2014) and, consequently, that post transfer refinement of microbial hosts is also widespread in nature.
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Engineered biosynthetic pathways in microbial hosts are often plagued by the metabolic imbalance that results from unregulated heterologous pathway construction.
Approaches and challenges for the production of these compounds from microbial hosts are discussed in this review.
In order to check whether the engineered microbial hosts were adequately engineered for sesquiterpene production, a selection of SeTPS genes was chosen from other plant species and demonstrated consistently high sesquiterpene titers.
Through novel implementations of ICO, CCO and MOCO, the high-expression genes of four microbial hosts were optimized and cross-validated to compare the performance of the optimized sequences.
These findings suggest that either identical microbial hosts are found in different environments or mobile viruses have broad host ranges (Jensen et al., 1998; Sullivan et al., 2003; Beumer & Robinson, 2005).
Another important aspect of large-scale fermentation using an engineered microbial host is the prevention of contamination.
Thus the searching of an optimal microbial host is still in process.
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