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The second is the annotation of microbial genomes using an integrated software pipeline, which analyzes sequence contigs and locates genomic regions that code for proteins, RNAs, and other genomic elements through the Do-It-Yourself Annotation (DIYA) framework [2].
We examined 75 complete microbial genomes using the Tm Index, and the analysis clearly differentiated hyperthermophilic from mesophilic microorganisms on this global genomic basis.
Searching microbial genomes using the Bcl-2 sequences or searching the human genome with T3SS sequences did not identify any sequence alignments with significant E-values.
De novo assembly of complete microbial genomes using new DNA sequencing technologies and without the aid of Sanger sequencing has been an unsolved challenge.
The second is the annotation of microbial genomes using an integrated software pipeline, which first analyzes contigs from high-throughput sequencing by locating genomic regions that code for proteins, RNA, and other genomic elements through the Do-It-Yourself Annotation (DIYA) framework.
The Rfam covariance models of each RNA motif were used to scan all selected microbial genomes using Infernal [ 37].
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Blast with microbial genomes used a value of 10 for expect and the default filter.
The authors are grateful to Angela Rennwanz who helped downloading the articles for the microbial genomes used in our analysis.
Primer specificity was determined by searching for similar sequences in microbial genome using the Basic Local Alignment Search Tool BLASTT).
The current study addressed the difficult issue of how to most effectively construct a complete microbial genome using today's state of the art sequencing technologies.
Thus, 220x coverage depth for Illumina and 150x coverage depth for SOLiD are considered good reference for optimizing experiment design in sequencing microbial genome using the hybrid assembly approach.
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microbial agents using
microbial populations using
microbial transcripts using
microbial communities using
microbial processes using
microbial strains using
microbial ecologists using
microbial samples using
microbial parameters using
microbial lipids using
microbial pathogens using
microbial fermenters using
microbial cells using
microbial genomes resources
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