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Genome mining of sequenced microbial genomes has resulted in a wealth of restriction enzymes with new specificities or unique properties (ApeKI (G∧CWGC), PhoI (GG∧CC), CviKI-1 (RG∧CY), NmeAIII (GCCGAG 20 21/18 19) [11], [11], Nt.CviPII (∧CCD) [12]; NEB catalog 2009/10) [1].
Comparison of completely sequenced microbial genomes has revealed how fluid these genomes are.
The availability of microbial genomes has allowed for new and remarkable insights into the evolution of microorganisms.
The total number of paralogs in microbial genomes has been shown to correlate with genome size [ 8].
But the evolution of our ability to manipulate microbial genomes has revolutionized the field of biotechnology and produced a rapid increase in innovation for industrial uses.
Although extensive investigation of BDGPs in mammalian and microbial genomes has been carried out, there are few publications regarding this phenomenon in plant genomes.
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Independently of Dr. Keim, however, the Institute for Genomic Research, which specializes in microbial genomes, had been determining the sequence of chemical building blocks in the DNA of the anthrax bacterium.
In the past few years, analyses of newly sequenced microbial genomes have revealed even more extensive gene jumping.
Several uncultured single microbial genomes have recently been sequenced using the single cell approach [15], [16], [17], [18].
Evaluation of intragenomic variation of the rRNA genes has become possible since more and more sequences of whole microbial genomes have recently become available.
Up to now, thousands of microbial genomes have been published in public databases.
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