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These unique motifs were used as query sequence to BLAST against the sequenced microbial genomes available in NCBI database (http://www.ncbi.nlm.nih.gov/), to validate the results.
However, despite the increasing number of microbial genomes available, genome comparison of several model strains may not fully represent the extreme variability of host specializations that can be found within one bacterial genus.
We downloaded the 277 microbial genomes available at the NCBI [ 32] on Nov. 03, 2005.
This website allowed manual annotation as well as orthology and synteny detection of microbial genomes available in NCBI.
We began by doing an all against all BLAST comparison of a collection of 617,000 proteins from all 214 completely sequenced microbial genomes available at the time.
A collection of 617,000 proteins was downloaded from 214 completely sequenced microbial genomes available on the NCBI ftp site in February 2005.
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Homologs of RecA and RpoB were retrieved from the Genbank NRAA database (ftp://ftp.ncbi.nih.gov/blast/db/FASTA/nr.gz) and from all complete microbial genomes publicly available in November, 2009 [51].
At the time of this implementation (June 2006), 268 complete microbial genomes were available through the National Center for Biotechnology Information (NCBI) and were downloaded from their ftp site [ 46].
BLASTP [ 18] searches were performed in summer 2008 against the approximately 650 complete or almost complete microbial genomes then available in the SEED database.
At the time of our implementation (June 2006), 268 complete microbial genomes were available through the National Center for Biotechnology Information (NCBI) and were downloaded from their ftp site [ 31].
More than two hundred complete microbial genomes are available to date in public databases and sequencing of a similar number is in progress [ 1].
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