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Thus, amplification of a microbial gene target produces a dual signal that allows assessment of specificity.
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MassTag PCR is a multiplex assay in which microbial gene targets are coded by a library of 64 distinct mass tags.
To address the need for sensitive multiplex assays in diagnostic molecular microbiology, we created a polymerase chain reaction (PCR) platform in which microbial gene targets are coded by a library of 64 distinct Masscode tags (Qiagen Masscode technology, Qiagen, Hilden, Germany).
The majority of probes (138 out of 143) produced hits only to the intended functional gene target within the same microbial genus.
Improved microbial gene identification with GLIMMER.
This microbial gene pool comprises genes from hundreds of microbial species.
While the representation of certain glycoside hydrolases in enriched deconstructive microbial communities provides promising gene targets, it offers no insight into whether communities actually express these genes.
In addition to measuring 31 traditional soil chemical and biological properties, we analyzed soil DNA using microarray technology to target those microbial genes involved in 11 specific ecosystem processes.
Soil DNA analysis using microarray technology can target those microbial genes involved in specific soil ecosystem processes and measure their abundance and diversity [26], [27], allowing a more complete investigation of soil quality.
In our hands, source tracking markers targeting enteric microbial genes of chicken and poultry showed low sensitivity and were not effective for detecting fecal contamination from poultry.
This approach mainly constrained the nutrient uptake rates to mimic the mature biofilm environment and performed single/double in silico gene knockouts to determine the gene targets essential for microbial growth in the biofilm.
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