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Our understanding of that vital microbial fraction of any holobiont and its influence on it continues to expand.
Real time PCR (qPCR) was initially performed on samples of the DNA extracted from the microbial fraction of Tampa Bay seawater to determine if the integrases from the viral metagenome were still present.
Although viruses are generally much smaller in size than their hosts, there are a number of reasons why viral sequences can be detected within the microbial fraction of seawater.
Since only a small proportion of GOS viral sequences were prophage-related, it appears that the majority of the viral sequences identified within the microbial fraction of data originated from actively replicating viral particles.
The metagenomic data generated from the microbial fraction of water samples was examined for the presence of viral sequences by comparison to the NCBI non-redundant (nr) protein database.
Furthermore, examination of viral sequences residing within the microbial fraction of GOS data revealed novel observations regarding distributional patterns of viral families over significant environmental gradients and potential interactions between highly abundant bacteriophage and host organisms.
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Our investigations of viral sequences identified within the microbial fractions of samples collected as part of the GOS expedition provided a unique opportunity to characterize microbial-associated viral populations over a large geographic transect.
The majority of the viral gene families in question (psbD, petE, speD, talC, pstS, and phoH) that were observed within the microbial fractions of samples were included in qPCR analyses.
Our coincident analysis of the microbial and viral sequences that were generated from the microbial fractions of environmental samples has provided us with a unique global perspective on the nature of (primarily) marine virus-host interactions and has stimulated intriguing questions with respect to the evolutionary trajectories of viruses and their hosts.
Taken together, the results of qPCR experiments indicate that the host-derived viral genes detected in the microbial fractions of samples are viral in nature and that viral genes encoding for environmentally significant, host-specific functions are incredibly prevalent in aquatic samples.
Our results suggest that, within the plant-adherent rumen microbial fraction, members of the Ruminococcaceae also have the greatest potential to produce cellulosome-like structures.
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