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Microbiological safety of food relies on microbial examination of raw materials and final products, coupled with monitoring process parameters and hygiene standards.
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The resident physicians have been trained the microbial examination as a part of our postgraduate medical education.
Furthermore, the microbial examination did not include specific tests for the detection of non-enteric viruses such as adenovirus.
Microbial examination was performed only on stool samples that were collected from children during routine clinical examination and analyzed.
Although microbial examinations have confirmed increased prevalence of atypical organisms in patients hospitalised after prior outpatient β-lactam treatment, 5 6 the present study does not extend this finding towards major clinical consequences when atypical pathogens are not covered in the initial inpatient antibiotic treatment.
Our initial approach to developing microbial biomarkers of exposure to bioavailable contaminants focused on examination of microbial molecular diversity along pollution gradients.
Whole genome amplification and sequencing of single microbial cells is a powerful new tool in the field of microbial genomics, enabling direct examination of the genomic contents of individual cells without the need of cultivation [1] [3].
The distinction between the examination of microbial burden on accountable and non-accountable surfaces proved to be of great importance in the data analysis.
An AFM instrument (Bruker Nano, Santa Barbara, CA, USA) and a silicon nitride probe with the calibrated spring constant of 0.7 N/m and tip radius of 10 nm were selected for the surface examination of microbial specimens.
The examination of microbial activity complemented those results obtained through chemical analysis, and when combined with hydrological data, showed that MLS samples provided a realistic profile of plume effects and could be related to the potential for natural attenuation of the site.
In addition to phylogenetic and statistical examination of microbial diversity in a single sample (i.e. alpha diversity), pyrosequencing has been used recently for comparative diversity studies, in which various aspects of microbial community composition is compared between multiple (few to hundreds) samples.
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