Compatibility of the rapid microbial enumeration method with existing microbial identification methods requires the ability to grow visible colonies (which are pure microbial cultures) from microcolonies.
Finally, some methods for rapid microbial enumeration require more hands-on labor than does the plate count method.
New rapid methods for microbial enumeration have been widely implemented in the healthcare and food industries.
Moreover, microbial enumeration was also performed by the spread plate count method using minimal selective solid medium (MSSM) with the same amount of AEC (30, 40, and 50%%, v/v) in order to ascertain their viability in the MSLM at the end of the experimentation.
For microbial enumeration, suspensions were plated using the microdilution plating method, on BHI agar plates, MRS agar plates or CASO agar plates (BHI/MRS/CASO broth: Sigma-aldrich, Diegem, Belgium; 15% agar: BD, Erembodegem, Belgium) and incubated at 37°C.
However, despite the high costs incurred by slow microbial testing and the availability of methods for faster testing [20], no rapid microbial enumeration technology has displaced a significant fraction of the more than 350 million traditional culture tests that are carried out by manufacturers of pharmaceuticals and personal care products each year [21].
Primarily culture-based enumeration methods have implicated LAB and Lactobacillus spp. in particular, as the dominant microbial contaminant during ethanol fermentation [ 1, 7, 8].
Different enumeration methods allow the analysis of Markush structures and their enumerated libraries.
Our goal in developing the technology described here is to provide a microbial enumeration test that is faster than visual plate counting, but maintains the advantages of the traditional method.
Microbial enumeration was done according to ISO 2718 (Technical Committee 1996).
