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Current SMGs that have been widely used in microbial engineering are antibiotic SMGs and auxotrophic SMGs.
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Effective microbial metabolic engineering is reliant on efficient gene transfer.
Genome-scale metabolic models (GEMs) can be used to evaluate genotype-phenotype relationships and their application to microbial strain engineering is increasing in popularity.
Relative to the introduction of gene(s) in a single organism wherein the genes depend on the regulatory- and metabolic network for proper expression, microbial consortium engineering is easier and achievable.
In this chapter, the commonly used strategies for microbial enzyme production and molecular engineering are systematically summarized, and we hope this review could make a contribution to the research and development of the microbial enzyme field.
Though denitrification is used as an example here, this synergy between microbial ecology and process engineering is applicable to other biological wastewater treatment processes.
Consequently, various microbial engineering strategies have been devised to endow producer microbes with tolerance phenotypes that would be required for economically viable production of the desired chemicals.
To transform microbial hosts into cellular factories, the applied discipline of systems metabolic engineering is using genome-scale approaches that redirect microbial metabolism to synthesize renewable and cost-effective biochemicals [ 1- 3].
Co-culture engineering is an emerging approach for microbial biosynthesis of a variety of biochemicals.
Transcription factor engineering is widely used to improve microbial strain tolerance against toxic molecules [ 12, 14].
The methods used in genetic engineering were developed in basic research of microbial genetics.
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