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Even though we live in a microbially dominated planet only the past decade the study of the microbial diversity has entered a period of considerable importance to science in general, industry, protection of the environment, and public policy making.
However, determining which species is responsible for what function can be an arduous task, since relatively little of the microbial diversity has been functionally characterized.
Lately, this pattern of recurrent discovery of novel microbial diversity has even suggested the existence of novel higher level groups, such as novel candidate domains of life.
Air microbial diversity has been recently studied using pyrosequencing technologies in the New York City subway platforms and associated sites [ 103].
The assessment of microbial diversity has shifted from primarily culture-based methods to approaches that make use of rapidly improving DNA sequencing technology.
While the use of richness and diversity parameters has still to be carefully considered, a decrease of evenness in structure of microbial diversity has been shown to be a good candidate warning indicator of process perturbation [ 43, 44].
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But both teams found that all the factors they were able to link to microbial diversity have less than a 20% impact, suggesting there's a lot more to be learned about our relationship with our bacteria.
Several technical limitations inherent to culture-independent explorations of microbial diversity have been highlighted [10].mong these, biases during DNA extraction and PCR amplification steps [12], primer selectivity, multiple rDNA gene copy number [13], and the existence of pseudogenes [14] or extracellular DNA [15], are particularly relevant.
In addition, both microbial cell concentration and microbial diversity have been seen to be elevated in sedimentary SMTZ environments [ 10].
Decreased microbial diversity have also been observed in other human diseases [ 39– 41], although increased diversity in diseased patients has also been noted [ 42].
Although individual taxa have been correlated with post-operative clinical course, global trends in microbial diversity have not been described in this context.
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