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This technique holds great promise for routine microbial diagnostics in laboratories.
Traditional culture-dependant techniques have been the mainstay of microbial diagnostics in CRS.
DNA microarrays have recently shown great potential in microbial diagnostics; in investigation of microbial diversity, composition and species identification from environmental and medical samples [ 7, 8].
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The described high level of achieved specificity and sensitivity demonstrates the potential and suitability of NASBA-microarray technology for the purpose of pathogen detection in microbial diagnostics or more complex analysis of microbial taxa in environment.
One explanation might be that emetic B. cereus strains are easily overlooked in routine diagnostic since they frequently show an atypical phenotype and might, in addition, be outcompeted on nonselective agar media often used in microbial diagnostics [ 32].
To combat this pressing issue, improved microbial diagnostics are urgently needed in resource-limited settings.
These characteristics make tmRNA a suitable marker molecule in microbial diagnostics.
All those characteristics make the tmRNA transcript (and its ssrA gene) a suitable tool as a target marker molecule for phylogenetical analysis and species identification in microbial diagnostics.
DNA microarrays have found several practical applications in microbial diagnostics such as composition analysis and species identification of different environmental and medical samples as well as in microbial diversity investigation [ 8- 10].
To test the SLICel designed probes for their potential use in microbial diagnostics; a new microarray was designed that consisted of the 25 best performing probes out of 97 according to their specificity and the sensitivity in the validation experiments.
A key characteristic of the NASBA-microarray technology, especially in microbial diagnostics, is that the detection and the identification of the correct target can be optimized at two different points in the experimental protocol.
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