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Of those, 341 (83%) had the results of all microbial determinations.
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However microbial determination was carried out with conventional methods for microbial detection.
All microbial resistance determinations, as well as analytical assays, were performed at least three times on different workings days.
The turves to be used for microbial biomass determination were frozen on the day of collection and were kept frozen during transport (ca. 2 weeks in total) prior to analysis.
Microbial analyses included determination of ergosterol and phospholipid fatty acid (PLFA) profiles.
This is a common characteristic of microbial ecologies, complicating determination and reporting of antimicrobial activity (Marsh 2004; Harrison et al. 2004; Sabater et al. 2007; Salli and Ouwehand 2015).
K-1 (phylloquinone) 0.075 During 4 weeks of experiment, faeces were collected and frozen at −70 °C for microbial enzymes activity determination.
As soon as possible after euthanasia (ca. 10 min), ileal, caecal and colonic pH were measured, sample of digesta was taken to determine dry matter, ammonia and SCFA and the rest were frozen at −70 °C for microbial enzymes activity determination.
For comparison, we quantified the abundance of bacterial amoA genes and estimated the portion of AOA and AOB in these BSC microbial communities using published determinations of archaeal 16S rRNA genes from the same samples (Soule et al.2009).2009
We conducted in-situ oxygen flux measurements in combination with ex-situ oxygen micro-profiles, respiration measurements, as well as rate determinations of microbial methane and sulfate turnover to assess respiration pathways as well as carbon turnover at a seep habitat that was recently discovered alongside the Hikurangi Margin offshore northern New Zealand.
We did not determine microbial C and N in forest litter since fumigation-extraction method was not applied for determination of microbial biomass in fresh organic materials [54].
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