Sentence examples for microbial culture methods from inspiring English sources

A major drawback of microbial culture methods is the length of time required for microbes to generate enough daughter cells to give rise to visible colonies.

Reliance on standard microbial culture methods limits detection of polymicrobial infections, especially anaerobic bacteria, and the routine use of anaerobic culture is labor-intensive and unreliable [8].

Neither P. aeruginosa nor S. marcesc ens was detected in the HPW using the developed NAD test or by traditional microbial culture methods.

Compared with biochemical, microbial culture methods and PCR assay (24 48 hrs, 3 hrs respectively); the ERM-LAMP is convenient, rapid, and sensitive.

The presence of both S. maltophilia and Burkholderia species in the HPW delivery system was also confirmed by traditional microbial culture methods using the BBL crystal enteric NF system (Becton Dickinson).

Therefore, monitoring water quality by HPCs enumeration with conventional microbial culture methods may only represent a relatively small proportion of the total viable microbial population in a biocontaminated water or HPW distribution system [ 25].

Previous studies have demonstrated that the microbial load present in water distribution systems can be significantly underestimated using the traditional plate count microbial culture method as a result of the presence of physiologically active bacteria that are unable to form colonies on culture media [ 23, 24].

In addition, a complete accounting of the microbiota is currently impossible, as there are no assays to detect all of >10 10 organisms in the intestines and standard microbial culturing methods miss 75 95% of these organisms.

Such studies demonstrate the need for and importance of real-time monitoring for the onset of biocontamination of HPW purification units and HPW delivery systems by rapid microbial culture independent methods such that prompt remedial action may be undertaken.

Hence, since the DEP measurement method eliminates the need for microbial culture, which is required within conventional diagnostic methods for enhancing their sensitivity toward viable versus nonviable C. difficile, the DEP velocity measurement method enables the quantification of microbial alterations at smaller antibiotic doses.

A variety of microbial assays were available during the search period (1985 2013), including documentation of the microbiota by either microbial cultures or metagenomic methods (16s rRNA-targeted probes using fluorescent in situ hybridisation (FISH) or other PCR technique) 8 21 28 31 or by indirect methods (Nugent scores).