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Since the acetate in our culture is directly oxidized to carbon dioxide, the system is better defined than the more complex, partly fermentative marine microbial community studied by Kraft et al. (2014), which even showed significant turnover of sulfate and sulfide.
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This work is focused on the development and application of functional gene arrays (FGAs) to target key functional gene markers for microbial community studies.
Our findings suggest that the results of PCR-based techniques used for microbial community studies should be compared to other methods to verify sensitivity.
The development of the new-generation sequencing technologies challenged the use of DNA microarrays in microbial community studies.
Recently, the development of the new-generation sequencing technologies challenged the use of DNA microarrays in microbial community studies.
Although frozen storage of biological samples is often necessary for archiving and logistic purposes, the potential effects on the number of taxa (composition) detected in microbial community studies are significant and should not be overlooked.
Environmental variables were monitored and the metabolic capabilities of the microbial community were studied using Biolog Ecoplates®.
The effect of the oil spill on the extant microbial community was studied using direct pyrosequencing.
PCR reactions were set up containing MegaMix ready PCR Mix (Microzone Ltd., UK) using the manufacturer's guidelines, and a fluorescently-labelled primer pair specific to the microbial community being studied (Table S1).
Here we present and validate the "double-RNA" approach for in-depth characterization of a soil microbial community by studying mRNA and rRNA molecules simultaneously from the same sample.
Methane-oxidising microbial communities are studied intensively because of their importance for global methane cycling.
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