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Microbial community profiles were determined by dilution plating on semi-selective media.
We characterized the soil microbial community structure through ester-linked fatty acid analysis (EL-FAME) and identified the soil environmental factors that best explain the pattern of microbial community profiles through canonical correspondence analysis (CCA).
Therefore, the objectives of this study were to characterize the effect of foliar glyphosate applied to GR (glyphosate resistant) soybeans on: (1) rhizosphere microbial community profiles using ester linked fatty acid methyl ester (EL-FAME) biomarkers, (2) exchangeable, non-exchangeable, and microbial K in the rhizosphere soil, and (3) concentrations of soybean leaf K.
A series of PCR-based analyses was then used to assess DNA extract quality and effect on microbial community profiles.
Xu et al., (2014) detected changes in dental plaque microbial community profiles and oral behavioral habits during the transition from a caries-free to a caries state using polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) in 3-year-old caries-free children followed up for 12 months.
However, there was no clear distinction between the microbial community profiles in healthy individuals and those with CCD.
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Segata, N. et al. Metagenomic microbial community profiling using unique clade-specific marker genes.
Results indicate that the communities changed rapidly after inoculation, but there was no drastic change in microbial community profile after 1 month.
Microbial community profiling of the pilot-scale systems revealed a greater proportion of known ammonia-oxidizing bacteria Nitrosomonas in (A) cells compared to (B) cells.
However, long-read sequencing is still costly, while short-read sequencing carries less information for complex microbial community profiling; therefore, the applications of high throughput sequencing platforms still remain challenging in microbial community reconstruction analysis.
The aim of this study was to evaluate the effects of ruminal and abomasum protein supplementation in cattle consuming tropical forages on nutritional characteristics, fibrolytic enzymatic activity, in vitro degradation of insoluble fiber, and the ruminal microbial community profile.
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