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Previous MFC microbial ecology studies have used Sanger-based 16S rRNA sequencing for microbial community characterization.
At the end of UASB operation, microbial community characterization revealed satisfactory composition of microbes whereas morphology depicted rod-shaped archaea.
This work first reviews recent literature on microbial community characterization in indoor environments (primarily those that utilized molecular methods), paying particular attention to the level of assessment of influential built environment characteristics and the specific methods and procedures that were used to collect those data.
Metagenomics is a powerful tool for viral and microbial community characterization since nucleic acids are isolated directly from environmental samples and sequenced, requiring no culturing, cloning, or a priori knowledge of what viruses may be present.
Using this more detailed microbial community characterization, it may be possible to move away from generic, single indicators to a community-indicator approach for assessing fecal contamination or environments conducive to pathogen growth.
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Compared to culture-based techniques, DNA sequencing of the bacterial 16S ribosomal RNA (rRNA) gene or whole metagenome shotgun (WMS) sequencing provides more precise microbial community characterizations.
In its simplest form, metagenomic analysis allows for a culture-independent characterization of microbial community composition.
Comprehensive samples of all genomic or transcriptomic data in a given microbial ecosystem, metagenomics, with recently developed software, can lead to accurate characterizations of microbial community function.
Comprehensive samples of particular genes or gene fragments, including amplicons, processed with recently developed software can lead to accurate characterizations of microbial community richness and diversity.
With the advent of metagenomic analyses, the latter may also involve molecular genetic characterization of the microbial community of the biofilm in the biofilter.
Provided the knowledge that only a very small fraction of microorganisms can be recovered in laboratory, this work aimed at a cultivation-independent characterization of the microbial community from a petroliferous basin area explored by PETROBRAS (Brazil), as well as from a randomly selected non-petroliferous area.
Related(16)
microbial community profile
microbial community identification
microbial community profiling
microbial population characterization
microbial community fingerprinting
microbial community characterized
microbial activity characterization
microbial community succession
microbial community structure
microbial community composition
microbial community analysis
microbial community variant
microbial community membership
microbial community shift
microbial community diversity
microbial community distribution
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