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Figure 4 Comparison of microbial communities based on 16S rRNA gene sequencing.
Differences were less pronounced for belowground organisms, i.e., nematode functional groups, microbial communities (based on phospholipid fatty acid (PLFA) analysis) and earthworm taxa.
The Integrated Microbial Genomes/Metagenomes (IMG/M) system also provides a collection of tools for functional analysis of microbial communities based on their metagenome sequence, based upon reference isolate genomes included from the Integrated Microbial Genomes (IMG) system and the Genomic Encyclopedia of Bacteria and Archaea (GEBA) project.
PCA demonstrated relative clustering of microbial communities based on presence or absence of NEC.
Assessment of antibiotic resistance in environmental microbial communities based solely on cultivable bacteria will therefore easily generate unrepresentative and biased results [20], [23] [23].
For all four airway sites, we confirmed that the trained models were better able to assign microbial communities based on smoking status than by guessing alone (P<2.2E-16 P<2.2E-16rwat sites, FriedmallRairwaym tesitesig. 3).
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Therefore, the prediction of the functional capabilities of a microbial community based on marker gene data would be highly beneficial.
Since the Illumina sequencing of 16S rRNA gene fragments in the metagenome produced no chimera or bias that is associated with the initial PCR step of amplicon sequencing, the taxonomic composition of the microbial community based on this sequencing method is highly reliable.
Phylogenetic analyses based on 16S rRNA gene sequences revealed that microbial communities found in the brines are not found in normal seawater [216].
The use of rRNA-based analyses to characterise active microbial communities is based on the assumption that active or growing cells have increased levels of rRNA relative to dormant, or intact dead cells.
(JPG 31 KB) 13568_2014_22_MOESM2_ESM.jpg Additional file 2: Figure S2.: Microbial community distribution based on the V3-V5 primer set in DPMM and DPME.
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microbial community based
microbial kingdoms based
microbial demands based
microbial genomes based
microbial risks based
microbial insecticides based
microbial surveys based
microbial communities associated
microbial processes based
microbial cultures based
microbial species based
microbial profiles based
microbial pathogens based
microbial communities involved
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