It is plausible to consider that microbial taxonomy will be steadily more dependent on genome sequences than relying on the classic polyphasic, including phenotypic characterization using time-consuming laborious laboratory tests.
Ribotyping of the isolates was performed using the Riboprinter Microbial Characterization system as previously described [ 15, 16].
Automated ribotyping was performed by using the Riboprinter Microbial Characterization System (DuPont Qualicon, Wilmington, DE), according to the manufacturer's protocol.
Ribotyping was performed using the automated RiboPrinter™ Microbial Characterization System (Qualicon Inc., DE, USA).
Ribotyping was performed by using the restriction enzyme EcoRI and the RiboPrinter Microbial Characterization System (Qualicon Inc., Wilmington, DE) as previously described (17, 18 ).
To further characterize the C. striatum isolates, we used 2 DNA fingerprinting techniques: automated ribotyping (RiboPrinter Microbial Characterization System; DuPont Qualicon, Wilmington, DE, USA) with EcoRI as restriction enzyme and pulsed-field gel electrophoresis (PFGE) macrorestriction analysis with 2 enzymes (XbaI and SwaI; New England Biolabs, Beverly, MA, USA).
The VITEK 2 microbial characterization system for biochemical characterization of the isolates and gram-negative and gram-positive cards was used to identify them.
For this purpose, 6 Motal cheese samples from Dasht-e-Moghan Dasht-e-Moghan Dasht-e-Moghanto micregion characterIranion.
For microbial characterization, 16S rRNA gene clone libraries and denaturing gradient gel electrophoresis (DGGE) fingerprinting were employed.
In this study, a comprehensive review of current knowledge in the microbial characterization, enrichment, and evaluation of effective parameters of microbial community in microbial electrolysis cells for typical biohydrogen production is summarized.
