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By pulsed electric field (PEF) application a permeabilization of plant, animal and microbial cells is achieved.
Isolation and separation of the target microbial cells is quite important in applied microbiology.
Density-gradient separation of microbial cells is highly selective, drastically modifying metabolic (CLP Profiles) and species (T-RFLP patterns) diversity, as well as activity parameters.
Highly efficient biosynthesis of the commercially valuable carotenoid astaxanthin by microbial cells is an attractive alternative to chemical synthesis and microalgae extraction.
The system is designed such that a sample is taken from the fermentor, the metabolism of microbial cells is instantaneously arrested, the cells are lysed in order to release intracellular compounds and in a subsequent binding assay the content of target molecules is monitored.
In conclusion, the main changing concept of using microbial cells is to use microbiota as a holistic integrity.
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Microbial cells are a developing technology that could potentially provide renewable energy (McCormick, 2011).
Microbial cells are attractive biorecognition elements for electrochemical biosensing applications.
Afterwards, the microbial cells were pelleted by centrifugation, and the supernatant was filtered through a 0.22-mm filter (Millipore).
Inside a biofilm, microbial cells are embedded in ECM, which binds them to each other and to the substrate.
Enzymes and microbial cells are increasingly being used for a variety of biochemical transformations.
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