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Twenty-four quinoxaline derivatives were evaluated for their antimycobacterial activity using BacTiter-Glo micellial cell viability assay.
Intracellular ATP production was measured using the BactTiter-Glo Microbial Cell Viability Assay kit (Promega) as described before [9].
Cell samples obtained in the cell growth study were analyzed using the BacTiter-Glo microbial cell viability assay kit (Promega) and bioluminescence was detected using a Promega Glomax luminometer.
The microbial cell viability assay was performed with a Microbial Viability Assay Kit-WST (Dojindo, Japan).
BacTiter-Glo Microbial Cell Viability Assay (BTG) (cat. no. G8232) was obtained from Promega.
Anti-infectives that target bacterial virulence or host immunity without affecting microbial cell viability offer an alternative to conventional antibiotics.
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Since cell growth is closely associated with ethanol production for many microorganisms including Z. mobilis (Delgenes et al. 1996; Zaldivar et al. 1999), it can provide relatively reliable estimates of the inhibitory effect of toxic compounds on microbial cells by measuring cell viability and growth with sensitive assays.
The most important factors for developing microbial starters include maintenance of cell viability, capacity for long-term storage and the drying method used.
Thus, both cellular-based microbial lung clearance and alveolar macrophage cell viability are decreased after chronic alcohol exposure and the resulting increase in oxidative stress (Velasquez et al. 2002).
For practical application of microbial cell arrays, cells on the array should maintain their viability and be able to be stored for sufficiently long periods of time.
While low to high intensity pulsing can enhance productivities, increased microbial exposure to ultrasonic power causes cells to become fragile and reduces microbial viability due to mechanical stress on the microbial cell [ 92].
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