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Radicle cross sections revealed microbial cell clusters abundant on the surfaces of plant cells, and sometimes clustering along the outer edges of the embryo (Figure 2B).
The microbial cell clusters were composed of ANAMMOX bacteria-like cells.
The filamentous bacteria departed from ANAMMOX subunits, and then gas tunnel developed in the interstitial space among microbial cell clusters.
Then the microbial cell clusters were assembled together with the help of filamentous bacteria to form an aggregate of cell cluster which is called ANAMMOX subunits.
Subunits, microbial cell clusters, or microbial cells were separated from each other by interstitial space which was known as interstitial voids.
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(iii) Microbial Cell Cluster.
(i) Formation of Microbial Cell Cluster.
According to the observation of Lu et al. [ 92] under light microscope and electron microscope, the structure of ANAMMOX granules included granule, subunit, microbial cell cluster, and single cell.
Thus researchers emphasized this and their microscopic observations assumed that granulation process accomplished with the sequential aggregation of microbial cells, cell clusters, and subunit.
In cell clusters microbial cells were aggregated close to each other by EPSs and filamentous bacteria, and the cluster size varied from few to 200 μm.
Light microscopy (Figure 3H) revealed melanized microbial cells resembling microsclerotia clustered within a developing bladder cell.
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