In the present study, we combined (1) statistical analyses of relative microbial abundance data based on extracted DNA and for comparison purposes from extracted RNA, (2) knowledge from literature related to the indicator genera identified, and (3) results from enrichment of pyrene degraders.
Currently, Seed requires two types of data, microbial abundance data and sample metadata.
The microbial abundance data contain counts or abundances of each microbial taxon in each sample.
The microbial rDNA abundance data obtained from analyzing weekly-withdrawn composted samples suggested population-shifts from bacteria-dominated to fungus-dominated communities.
Predefined sample groups (selected times of incubation as well as pyrene treatment versus controls) were statistically evaluated based on microbial genera relative abundance data and related to the degradation kinetics.
Tung J, Barriero LB, Blekhman R, Archie EA, 2015, Data from: Sample information and tables with microbial and genic relative abundance data, 10.5061/dryad.8gp03, Available at Dryad Digital Repository under a CC0 Public Domain Dedication.
Results obtained through pyrosequencing were subsequently compared to abundance data of selected microbial taxa identified through fluorescence in situ hybridization (FISH) in another study (Pérez et al. 2014) in order to assess the utility and potential limitations and biases of each method in analyzing microbial populations from biofilter samples.
Schmitt, S., Deines, P., Behnam, F., Wagner, M. & Taylor, M. W. Chloroflexi bacteria are more diverse, abundant, and similar in high than in low microbial abundance sponges.
Differences in the relative abundances of microbial groups between sample types were assessed by the two-tailed Student's t-test for unequal variance (logit-transformation of abundance data did not alter the reported results for untransformed data).
Microbial abundance profiles of 39 Indian subjects, showing inter-individual variations of microbial community structure.
The present study indicates an inverse relationship between microbial diversity and Helicobacter abundance as well as probable modulations of microbial abundance by Helicobacter, similar to those reported previously2,5.
