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However, some isolates are difficult to identify by those platforms, which are called "difficult-to-identify" microbes in this study.
However, some isolates are difficult to identify by the automated biochemical text platforms, which are called "difficult-to-identify" microbes in this study.
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First, though we used sequencing of 16S rDNA amplicons to predict the taxonomic identities of microbes cultured in this study, these results did not coincide with all of the TRFLP signals observed, nor can one assume that a TRFLP signal represents a single microbial species, because different bacteria can produce the same 16S rDNA restriction polymorphism.
These observations therefore, agree with the prolonged release behavior of curcuminoids and their potential against number of microbes as observed in this study.
There are a few important issues that need to be considered when generating and using mutant libraries for microbes as described in this study: (1) Two copies of the transposon may be integrated in vitro into the same target DNA fragment, although rarely, as the reaction stoichiometry favors single insertions.
The combination of complete genomic sequence data, genetic tractability, and sophisticated analytical tools for D. melanogaster and P. fluorescens will facilitate future characterization of the cellular, molecular and developmental components of the host-microbe interactions observed in this study.
This strain was originally isolated from human saliva and is used as a model microbe in the study of host-microbe interactions, due to its established taxonomy, its ability to survive gastrointestinal (GI) transit [ 8] and its putative role as a probiotic species [ 9].
Infections due to other multidrug-resistant microbes in our study can mainly be considered healthcare-associated.
In addition, in this study, the microbes taking part in the reduction of Congo red were also identified by PCR-DGGE approach.
The average total viable count (microbial load) showed the presence of microbes in all the drinks analyzed in this study in the range of 7.7 × 103 - 9 × 108 cfu/ml.
Despite the fact that MIP has been found to be highly resistant against isoniazid (INH) in an in vitro assay model, in this study the microbe was highly susceptible to this standard anti-TB drug.
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