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In our work, we fabricated the microarrays with the five ToRCH-related antigens and screened 100 specimens of randomly selected clinical sera from outpatients along with quantum dots-labeled secondary antibody.
Firstly, we fabricated the microarrays with the five kinds of ToRCH-related antigens and used CdTe quantum dots to label secondary antibody and then analyzed 100 specimens of randomly selected clinical sera from obstetric outpatients.
Thus this technique combines the genome-wide scale of microarrays with the transcript variant detection power of Expressed Sequence Tags (ESTs).
One microgram of total RNA (control and test) samples was amplified and labeled to generate cDNA for oligo microarrays with the Agilent low RNA input linear amplification kit (Cat. No 5184 3523).
In summary, Mab-seq combines the relative ease and low-cost of microarrays with the power and depth of HTP sequencing to create flexible, quality-tested libraries that can be archived and re-used multiple times.
ADOT combines custom oligonucleotide microarrays with the S9.6 antibody to identify chromosomal translocations in cancer.
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With 10 designed probes, ALV-A, ALV-E, ALV-J, REV, MDV pathogenic and vaccine strains were clearly discriminated on the microarray with the naked eyes.
We analyzed the hybridization pattern of this constructed microarray with the Cy3-labeled genomic DNA of various food-borne pathogens and other bacteria.
We compared those genes identified by microarray with the reported MiTF and TFEB targets [36], [35].
Expression changes measured by PCR were comparable to those measured by microarray, with the median difference between microarray versus RT-PCR ratios being 11% (Supporting Dataset S2).
This strain is indistinguishable from WA MRSA-55/56 in all markers covered by the microarray with the sole exception of the SCCmec element.
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