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The microarrays were validated on DNA extracted from reference strains and used to type 24 enterococcal strains belonging to various species and with different resistance phenotype, detecting all the genes of the resistance clusters which were actually present in each control and study strain.
The expression profiles obtained using custom Agilent microarrays were validated by quantitative RT-PCR.
Taken together, these results show that most of the genes identified by microarrays were validated and only few genes were found to be false positive results.
The microarrays were validated by qRT-PCR assay.
The results obtained with the microarrays were validated for all genes tested.
Sixteen additional genes, over-expressed on microarrays were validated using qRT-PCR.
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In addition, up-regulation on microarrays was validated by independent qPCR on arbitrarily selected genes.
The differential expressions of 19 randomly selected genes identified by microarray were validated.
Seven significant genes found by microarray were validated by PCR.
The results of the microarray were validated by real-time PCR.
Data from microarray were validated by qPCR in twenty pairs of samples.
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