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The accuracy and reliability of the results obtained with microarrays were tested by quantitative RT-PCR (Q-PCR) of 10 selected genes belonging to different functional classes.
To validate results obtained by microarrays, nine genes (PHLDA2, FERMT2, RP2, SHISA2, MCF2L, TGFBR3, SLC11A2, SERPINC1, FDFT1) found differentially expressed by microarrays were tested using real-time RT-PCR.
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Finally, the reliability of using a universal RNA reference for two-channel microarrays was tested and the results suggest that comparisons of multiple experimental conditions using the same control can be accurate.
The accuracy and reliability of the results obtained with microarrays was tested by quantitative RT-PCR (Q-PCR) using a tailored TaqMan Low Density Array (LDA).
The expression of a total of 32 genes identified by the microarrays was tested by realtime PCR.
In addition, all serial samples from women who had autoantibodies to p53 peptides in the microarray were tested.
The variance differences between Cy3 and Cy5 intensities within the microarray were tested with the Student's t-test under the assumption of both uniform and non-uniform variances.
The microarray was tested against 16 Salmonella strains of known serotype.
The availability of the microarray was tested using seawater samples from shrimp ponds, and the results were validated by pyrosequencing.
The microarray was tested on mussels exposed to sublethal concentrations of mercury or a crude North Sea oil mixture.
The Pinus microarray was tested by heterologous hybridisation with cRNA produced from bark, mucilaginous xylem and xylem of P. radiata clonal saplings that were subjected to an ethephon induction trial.
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