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Orthologous genes between mouse and human microarrays were selected using HomoloGene database of National Center for Biotechnology Information (NCBI).
Only probe sets that had at least an intensity of 20 and a present call at one of the microarrays were selected.
Breast cancer tissue microarrays were selected based on the availability of paraffin blocks and prepared by extracting two 1-mm diameter cores of histological confirmed invasive breast carcinoma and immunohistochemically stained for ER, PR, basal cytokeratins HER2, CK5/6 and vimentin.
Then, the 15 candidate miRNAs discovered via microarrays were selected for further testing by qRT-PCR in the training phase.
The cDNA clones used for both microarrays were selected from the cDNA libraries generated by the Sino-Danish Pig Genome Sequencing Consortium [ 14].
Genes presenting a mean intensity above the cutoff value on one of the six microarrays were selected for further analysis: 1709 genes were selected.
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A set of genes identified by the microarrays was selected for further analysis by immunohistochemistry and immunofluorence staining to confirm and explore corresponding protein levels and cell type expression in skin.
Two species highly expressed at 3H by the microarray were selected for western blot analysis.
A total of 27 genes with different levels of expression detected using microarray were selected for real-time PCR verification using the same RNA purified for microarray hybridizations.
Candidates identified from the comparison of follicular tissues from the microarray were selected using a multi-level filtering system.
Sequences targeted by the microarray were selected for quantitative PCR analysis to corroborate the results obtained in the microarray (Table 3).
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