After the microarrays were air-dried, the results were recorded by microarray scanner (GenePix 4200A, Molecular Devices, Sunnyvale, CA) at 5 μm resolution.
For molecular interaction and pathway analyses of selected miRNAs, previously validated target genes from degradome sequencing [ 28, 40, 60] and microarray data [ 61] were recorded and their molecular interactions were illustrated by Cytoscape 2.8.2 [ 80].
Finally, allergens with >50% missing data were discarded and results were recorded in microarray units corresponding to the Tukey mean of corrected fluorescence intensity of replicates.
Genome-wide transcriptional changes triggered by diamide, H2O2 and menadione in A. nidulans vegetative tissues were recorded using DNA microarrays containing 3533 unique PCR-amplified probes.
By means of microarray hybridization, high-definition methylation profiles were recorded covering thirty-three CGIs associated with the twenty-eight selected genes.
Genome-wide transcription profiles of the wild-type and the sigB mutant were recorded by comparative DNA microarray hybridizations.
Similar changes were recorded by Q-PCR and microarray for Tiam1 (566/106) and Jmjd4 (98/145).
Behavioral data were recorded, whole hypothalami were collected, and microarray hybridizations were performed.
Fluorescence intensities were recorded, and data processing was performed by using the Microarray Image Analysis software (Phadia).
Raw data of the performed microarray experiments have been recorded in the GEO public database (accession number: GSE34929).
